Laboratory Research Technician III in Pittsburgh, Pennsylvania at University of Pittsburgh

Laboratory Research Technician III

Med-Surgery - Pennsylvania-Pittsburgh - (26004422)

The Pinzon Guzman is seeking a Research Technician III. The successful candidate will have prior basic science research experience. The Research Technician III will work closely with other lab members (such as Post Docs on high priority experiments. The Research Technician III will be required to perform moderately complex research activities while playing an important role in facilitating day-to-day operations of the lab. The Candidates will work on various projects such as: “Restoring the BMP/Noggin Balance in Esophageal Atresia: Integrating Pathogenesis with Surgical Innovation to Improve Outcome.”

Job Summary

Performs a variety of independent laboratory work and experiments such as determining experimental protocols and preparation of specimens and cultures. Prepares reports of findings subsequent to compilation and analyzation. Oversees specimen inventory. Develops documentation for publication; crafts manuscripts and grant proposals.

Essential Functions

Responsibilities: Human cell culture (routine handling, transfection, flow cytometry) and other standard bench work like Western Blot. DNA isolation from cells, PCR amplification. Maintaining laboratory stocks of lab supplies, reagents, buffers, plates, etc. and order new supplies as needed. Performing routine maintenance of laboratory instruments. Assisting in analyzing and summarizing data for scientific communications (e.g., grant applications, peer-reviewed publications). Maintaining records of experimental data and communicating results to lab members. Reviewing pertinent scientific literature. Other duties as assigned.

Must have an understanding of basic molecular biology and genetics. Must have excellent verbal and written communication skills. Theoretical and practical knowledge of molecular cloning techniques is essential (DNA/RNA isolation, restriction digestion, PCR amplification, purification, etc.). Experience with Organoids. Experience with the proper handling and genetic manipulation of bacteria is essential. Proficiency with basic computational tools. Experience with flow cytometry and FACS would be an asset.

Physical Effort

Physical effort associated with wet lab work, i.e. standing while conducting experiments, sitting while working on computer, walking back and forth in the lab to sinks and microscopes as well as occasionally going to the loading doc to pick up supplies, lifting packages less than 20lbs., etc.

Assignment Category: Full-time regular

Job Classification: Staff.Laboratory Research Technician III

Job Family: Research

Job Sub Family: Laboratory

Campus: Pittsburgh

Minimum Education Level Required: Bachelor's Degree

Minimum Years of Experience Required: 2

Will this position accept substitution in lieu of education or experience: Combination of education and relevant experience will be considered in lieu of education and/ or experience requirement.

Work Schedule: Monday-Friday, 8:00 am - 4:30 pm

Work Arrangement: On-Campus: Teams that work on campus, in an office, or in a lab.

Hiring Range: To be determined based on qualifications

Relocation_Offered: No

Visa Sponsorship Provided: No

Background Check: For position finalists, employment with the University will require successful completion of a background check

Child Protection Clearances: The following PA Act 153 clearances and background checks are required prior to commencement of employment and as a condition of continued employment: PA State Police Criminal Record Check, FBI Criminal Record Check, PA Child Abuse History Clearance.

Required Documents: Resume

Optional Documents: Cover Letter